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Evidence abounds that young stellar objects undergo luminous bursts of intense accretion that are short compared to the time it takes to form a star. It remains unclear how much these events contribute to the main-sequence masses of the stars. We demonstrate the power of time-series far-infrared (far-IR) photometry to answer this question compared to similar observations at shorter and longer wavelengths. We start with model spectral energy distributions that have been fit to 86 Class 0 protostars in the Orion molecular clouds. The protostars sample a broad range of envelope densities, cavity geometries, and viewing angles. We then increase the luminosity of each model by factors of 10, 50, and 100 and assess how these luminosity increases manifest in the form of flux increases over wavelength ranges of interest. We find that the fractional change in the far-IR luminosity during a burst more closely traces the change in the accretion rate than photometric diagnostics at mid-infrared and submillimeter wavelengths. We also show that observations at far-IR and longer wavelengths reliably track accretion changes without confusion from large, variable circumstellar and interstellar extinction that plague studies at shorter wavelengths. We close by discussing the ability of a proposed far-IR surveyor for the 2030s to enable improvements in our understanding of the role of accretion bursts in mass assembly.

 

Machine tasks in workshops or factories are often a compound sequence of local, spatial, and body-coordinated human-machine interactions. Prior works have shown the merits of video-based and augmented reality (AR) tutoring systems for local tasks. However, due to the lack of a bodily representation of the tutor, they are not as effective for spatial and body-coordinated interactions. We propose avatars as an additional tutor representation to the existing AR instructions. In order to understand the design space of tutoring presence for machine tasks, we conduct a comparative study with 32 users. We aim to explore the strengths/limitations of the following four tutor options: video, non-avatar-AR, half-body+AR, and full-body+AR. The results show that users prefer the half-body+AR overall, especially for the spatial interactions. They have a preference for the full-body+AR for the body-coordinated interactions and the non-avatar-AR for the local interactions. We further discuss and summarize design recommendations and insights for future machine task tutoring systems. 

The dynamic rearrangement of the actin cytoskeleton is an essential component of many mechanotransduction and cellular force generation pathways. Here we use periodic surface topographies with feature sizes comparable to those of in vivo collagen fibers to measure and compare actin dynamics for two representative cell types that have markedly different migratory modes and physiological purposes: slowly migrating epithelial MCF10A cells and polarizing, fast-migrating, neutrophil-like HL60 cells. Both cell types exhibit reproducible guidance of actin waves (esotaxis) on these topographies, enabling quantitative comparisons of actin dynamics. We adapt a computer-vision algorithm, optical flow, to measure the directions of actin waves at the submicron scale. Clustering the optical flow into regions that move in similar directions enables micron-scale measurements of actin-wave speed and direction. Although the speed and morphology of actin waves differ between MCF10A and HL60 cells, the underlying actin guidance by nanotopography is similar in both cell types at the micron and submicron scales. 

Oral delivery, while a highly desirable form of nanoparticle‐drug administration, is limited by challenges associated with overcoming several biological barriers. Here, the authors study how fluorescent and poly(ethylene glycol)‐coated (PEGylated) core‐shell silica nanoparticles sized 5 to 50 nm interact with major barriers including intestinal mucus, intestinal epithelium, and stomach acid. From imaging fluorescence correlation spectroscopy studies using quasi‐total internal reflection fluorescence microscopy, diffusion of nanoparticles through highly scattering mucus is progressively hindered above a critical hydrodynamic size around 20 nm. By studying Caco‐2 cell monolayers mimicking the intestinal epithelia, it is observed that ultrasmall nanoparticles below 10 nm diameter (Cornell prime dots, [C’ dots]) show permeabilities correlated with high absorption in humans from primarily enhanced passive passage through tight junctions. Particles above 20 nm diameter exclusively show active transport through cells. After establishing C’ dot stability in artificial gastric juice, in vivo oral gavage experiments in mice demonstrate successful passage through the body followed by renal clearance without protein corona formation. Results suggest C’ dots as viable candidates for oral administration to patients with a proven pathway towards clinical translation and may generate renewed interest in examining silica as a food additive and its effects on nutrition and health.

 

Acquiring accurate high temperature laboratory‐based infrared emission spectra of geologic samples is important to constrain their radiative and spectral properties. This is important in calculations of lava flow cooling, crust formation, and ultimately lava flow propagation modeling. However, measuring accurate emission at high temperatures remains a challenge. A new micro‐furnace design was created to integrate with a Fourier transform infrared spectrometer, replacing the previous furnace and improving the performance and error metrics. Importantly, this approach accounts for all significant error sources and uses only one spectrometer to acquire sample and calibration emission data over greater temperature (473–1,573 K) and spectral (4,000–500 cm⁻¹, 2.5–20 μm) ranges. Emissivity spectra of forsterite and quartz samples were acquired to test the calibration procedure. Forsterite, with no expected phase transitions over the temperature range, showed spectral change above ∼1140 K, potentially due to amorphization–a process not well described in past studies. The quartz results revealed the expected polymorph transformations at ∼846 and ∼1323 K. A Hawaiian basalt sample served as a representative rock test and showed an increase in emissivity (∼25%) with decreasing temperature. The greatest emissivity increase (∼60%) occurred in the middle infrared region (3,333–2,000 cm⁻¹, 3–5 μm). This is significant for thermal/mass flux calculations using satellite data in this spectral region, which rely on emissivity to derive accurate temperatures. All results are consistent with our previous investigations, but with improved mean accuracy (<2%), uncertainty (<4%), and spectral contrast (<20%). The improved metrics were achieved by constraining the sample measurement geometry, sample temperature stability, and environmental contamination within the experiment.

 

Dot1 (disruptor of telomeric silencing-1), the histone H3 lysine 79 (H3K79) methyltransferase, is conserved throughout evolution, and its deregulation is found in human leukemias. Here, we provide evidence that acetylation of histone H4 allosterically stimulates yeast Dot1 in a manner distinct from but coordinating with histone H2B ubiquitination (H2BUb). We further demonstrate that this stimulatory effect is specific to acetylation of lysine 16 (H4K16ac), a modification central to chromatin structure. We provide a mechanism of this histone cross-talk and show that H4K16ac and H2BUb play crucial roles in H3K79 di- and trimethylation in vitro and in vivo. These data reveal mechanisms that control H3K79 methylation and demonstrate how H4K16ac, H3K79me, and H2BUb function together to regulate gene transcription and gene silencing to ensure optimal maintenance and propagation of an epigenetic state.